Nikon 80i Microscope Fluorescence Phase Contrast Pred Ni-U
Setup for Brightfield Transmitted with Fluorescence Reflective and Phase Contrast studies.
This Nikon 80i Microscope is in good working order with all aspects tested and fully working.
Microscope optics have been cleaned professionally by our technician under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Nikon 80i Microscope passed all tests and was found to be fully functional:
Tested halogen lamp and source: PASSED
Tested mercury lamp and source: PASSED
Tested mechanical XY stage to limits: PASSED
Tested Z-axis focus to bring sample into focus: PASSED
Tested connection between microscope camera and computer: PASSED
Tested live feed on computer: PASSED
Tested iris adjustment: PASSED
Tested all buttons and controls: PASSED
Tested both lightpaths and shutters: PASSED
During testing this Nikon 80i Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the motorized components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Nikon Eclipse 80i Upright Microscope
(2) CFI 10x/22 Eyepieces
0.90 DRY Condenser
Prisms: Ph1, Ph2, Ph3
Built-in Illumination Tower w/ shutters
D-DH Fluorescence Lightpath w/ manual shutters
Fluorescence Filter Cubes:
UV-2E/C EX 350/50nm DM 400lp EM 460/50nm
B-2E/C EX: 480/30nm DM: 505nm EM: 535/40nm
Y-2E/C EX: 560/40nm DM: 595lp EM: 630/60nm
D-LH 12V 100W Halogen Lamp with built-in power supply
LH-M100CB-1 100W Mercury Lamp with HB-10103AF power supply
C-SR Mechanical XY Stage
6-position Mechanical Nosepiece
4x/0.13 Plan Fluor
10x/0.30 Plan Fluor Ph1 DL
20x/0.75 CFI Plan Apo VC (this is a high quality Nikon APO CFI60 Objective, it was originally installed in a custom piece of equipment where manufacturer did not want to show that they used Nikon objectives)
Photo / Port Splits:
100% Eye or 100% Camera
AmScope MU300 3.1MP Digital Color Camera
Computer and Software:
Dell Latitude i5 Laptop running Windows 10 with AmScope software
Communication and power cables.
Configuration and Setup:
Illumination Type: Brightfield with Phase Contrast and Fluorescence
Objectives: 4x/0.13 Plan Fluor, 10x/0.30 Plan Fluor Ph1 DL, 20x/0.75 CFI Plan Apo VC
Light Sources: 12V 100W Halogen Lamp, 100w Mercury Lamp
Stage: Mechanical XY
This Nikon 80i Microscope is currently set up for Brightfield viewing with Phase Contrast and Fluorescence studies.
This Nikon system includes a Dell i5 Laptop running Windows 10 with AmScope Micromanager software.
With the included 4x, 10x,and 20x combined with 10x eyepieces the total magnification is 200x.
This video beings with a full front view of the Nikon 80i Microscope powered up and running. The camera scans from right to left for a closer look.
Next we see the AmScope live feed with a demo via the focal control.
Next we see the XY control and finally the fluorescence before a final front view of the Nikon 80i Microscope ends this video.
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