Here is a Leica DM5500 B Fluorescence Motorized Phase Contrast Microscope DM5500B
Made in 2009
Setup for Brightfield with Fluorescence and Phase Contrast studies.
Current models are DM4 B and DM6 B
This Leica DM5500 B Fluorescence Motorized Phase Contrast Microscope is in good working order with all aspects tested and working fully.
Microscope optics have been cleaned professionally by our technician under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Leica DM5500 B Fluorescence Motorized Phase Contrast Microscope passed all tests and was found to be fully functional:
Tested halogen lamp source fully for intensity and power: PASSED
Tested fluorescence lamp and source fully: PASSED
Tested port splitter to split between eyepieces and camera port: PASSED
Tested connection from computer to cameras for live feed: PASSED
Tested control of the motorized XY stage w/ joystick: PASSED
Tested motorized XY stage to limits: PASSED
Tested microscope touchscreen to control all motorized parts: PASSED
Tested objectives for clarity and focus: PASSED
Tested eyepieces for clarity: PASSED
Tested filters and prisms: PASSED
Tested all power switched and light adjustments on microscope: PASSED
Tested all motorized components including: XY stage, Z-axis focus, filter cube turret, condenser, fluorescence shutter, halogen shutter and aperture: PASSED
During testing this Leica DM5500 B Fluorescence Motorized Phase Contrast Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Leica DM5500 B Upright Microscope
CTR5000 Control Box/ Power Supply
(2) L PLAN 10x/20 Eyepieces
Prisms / Condensers:
7-position Motorized Condenser 0.90 S1 Lens
Fluorescence Light Path:
Built-in Motorized Fluorescence Turret
DAPI/Green/Orange triband filter cube EX 390,475,560nm DM 425, 525, 610nm, EM 430, 530, 615nm
12V 100W Halogen Lamp w/ CTR5000 Control Box/ Power Supply
EXFO X-Cite 120Q Metal Halide Light Source w/ light guide
PRIOR Motorized Stage XY
PRIOR ProScan II Control Box
PRIOR Joystick CS152V2
6-position Smart Objective Turret
10x/0.30 HC PL FLUOTAR
20x/0.40 L N PLAN CORR PH1
40x/0.75 PL APO
Photo / Port Splits:
100% Eye or 100% Camera
AmScope MU1003 10.0MP USB 3.0 CMOS camera
Computer and Software:
Dell Latitude I5 Laptop running Windows 7 Pro with AmScope software
XY Stage, Z-axis Focus, Filter Cube Turret, Condenser, Fluorescence Shutter, Halogen Shutter and Aperture.
Communication and power cables.
Configuration and Setup:
Illumination Type: Brightfield w/ Fluorescence and Phase Contrast
Objectives: 10x/0.30 HC PL FLUOTAR, 20x/0.40 L N PLAN CORR PH1, 40x/0.75 PL APO
Light Sources: 12V 100W Halogen, EXFO X-Cite Metal Halide
Stage Type: Motorized XY
This Leica DM5500 B Fluorescence Motorized Phase Contrast Microscope is currently set up for Brightfield viewing with Phase Contrast and Fluorescence studies.
The AmScope MU1003 10MP camera installed is used for CMOS colored imaging.
The AmScope camera/software is capable of simple measurement techniques of samples.
This video begins with a full front view of the Leica DM5500 B Fluorescence Motorized Phase Contrast Microscope powered up and running.
The camera pans from right to left for a closer look. Next we see the AmScope live feed and software information.
A demonstration is given to with the motorized XY and focus. Finally we see the Fluorescence as it effects the light on the sample.
A final front view of the Leica DM5500 B Fluorescence Motorized Phase Contrast Microscope will end this video.
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